Staining of SJL spenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 μg of Cy5 anti-mouse CD45.1 (A20) (colored histogram). Cells in the lymphocyte gate were used for analysis.
Contents: Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1) Catalog Number: 19-0453 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: A20 Host/Isotype: Mouse IgG2a, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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19-0453-81
50 ug
19-0453-82
100 ug
19-0453-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The A20 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The A20 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
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