Product Information

Contents: Horseradish Peroxidase (HRP) Goat anti-rat IgG
Catalog Number: 18-4818
Sizes: 100 ug
Formulation: 0.01 M Sodium Phosphate, 0.25 NaCl, pH 7.6 with 15 mg/ml BSA (IgG-Free, Protease-Free)
Storage Conditions: For extended storage as a liquid, add an equal volume of glycerol (ACS or better grade) for a final concentration of 50% followed by storage at -20°C. Please note that the concentration of protein and buffer salts will be one-half of the original after the addition of glycerol. Alternatively, the product may be aliquoted and frozen at -70°C or below, in the absence of glycerol. It is important to avoid repeated freezing and thawing. This product is guaranteed one year from date of receipt.
Clone: Polyclonal

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
18-4818	Horseradish Peroxidase (HRP) Goat anti-rat IgG	N/A	N/A	ELISA IHC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This is affinity purified HRP-conjugated Goat Anti-Rat IgG (H+L). This antibody has minimal cross-reactivity to Mouse, Human, Bovine, Horse, and Rabbit Serum Proteins. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rat IgG and with the light chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Applications Reported

For research use only, not for diagnostic or therapeutic use. HRP anti-rat IgG has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA.

Applications Tested

This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human, bovine, horse, and rabbit serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Suggested dilution ranges are:
1:500 - 1:5,000 for immunohistochemistry on tissue sections
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Related Products

Cat. 00-0000    eBioscience 10X ELISA Coating Buffer
Cat. 44-2404    Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 18-4100    Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202    eBioscience ELISA Diluent Solution, 5X
Cat. 13-4213    Biotin Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)