Product Information

Contents: Horseradish Peroxidase Avidin D (HRP-AV)
Catalog Number: 18-4100
Sizes: 1 ml, 500 ul
Formulation: PBS/BSA, 0.2% ProClin 150
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
DO NOT USE SODIUM AZIDE.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
18-4100	Avidin Horse Radish Peroxidase (AV-HRP)	N/A	N/A	ELISA IHC WB

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Description

The avidin horseradish peroxidase is commonly used as a second step for detection of biotinylated antibodies by ELISA and immunohistochemical staining. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]

Applications Reported

For research use only, not for diagnostic or therapeutic use. Avidin-HRP has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA.

Applications Tested

This lot of AV-HRP has been tested by ELISA to detect biotinylated primary antibodies and is recommended to be diluted in ELISA Diluent Solution (cat. 00-4202) at 1:1000. The recommended dilution for use in Western Blot is 1:500-1:1000. It is recommended that this product be carefully titrated for optimal performance in the assay of interest.