Product Information

Contents: Allophycocyanin (APC) anti-human CXCR4 (Fusin, CD184)
Catalog Number: 17-9999
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 12G5
Isotype: Mouse IgG2a, κ

Staining of human lysed whole blood with FITC anti-human CD19 (HIB19) (cat. 11-0199) and APC Mouse IgG2a, K Iso Cntrl (cat. 17-4724) (left) or APC anti-human CXCR4 (12G5) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9999	Affinity Purified anti-human CXCR4 (Fusin, CD184)	N/A	N/A	FA FC
16-9999	Functional Grade* Purified anti-human CXCR4 (Fusin, CD184)	N/A	N/A	FA FC
13-9999	Biotin anti-human CXCR4 (Fusin, CD184)	N/A	N/A	FC
12-9999	PE anti-human CXCR4 (Fusin, CD184)	488	575	FC
15-9999	PE-Cy5 anti-human CXCR4 (Fusin, CD184)	488	670	FC
17-9999	Allophycocyanin (APC) anti-human CXCR4 (Fusin, CD184)	633	660	FC
25-9999	Phycoerythrin-Cy7 (PE-Cy7) anti-human CXCR4 (Fusin, CD184)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 12G5 monoclonal antibody reacts with human CXCR4 (CD184), also termed Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4⁺ cells. CXCR4 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. 12G5 shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CXCR4), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 12G5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of human lysed whole blood. This can be used at 20 μl (0.25μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

McKnight, A. et al. (1997). "Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent." J Virol 71(2): 1692-6.
Bleul, C. et al. (1997). "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." Proc Natl Acad Sci U S A 94(5): 1925-30.

Related Products

Cat. 17-4724 Allophycocyanin (APC) Mouse IgG2a, K Isotype Control