Anti-Human HLA-G APC

RUO: For Research Use Only

human HLA-G antibody, Allophycocyanin (APC) conjugate

Staining of the U937 cell line stimulated with GM-CSF and IFNγ with Mouse IgG2a κ Isotype Control APC (cat. 17-4724) (blue histogram) or Anti-Human HLA-G APC (purple histogram). Green histogram represents staining with Anti-Human HLA-G APC on unstimulated cells. Total viable cells were used for analysis.

Contents: Anti-Human HLA-G APC
Catalog Number: 17-9957
Concentration: 5 µl (0.125 µg)/test
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material.
Clone: 87G
Host/Isotype: Mouse IgG2a

Product Options
Cat. No.	Size	Price	Add Qty to Cart
17-9957-41	25 tests	To view relevant pricing, please select your country and resume browsing.
17-9957-42	100 tests

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
00-4222	Flow Cytometry Staining Buffer
17-4724	Mouse IgG2a K Isotype Control APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-9957	Anti-Human HLA-G PE	488	575	FC	RUO
14-9957	Anti-Human HLA-G Purified	N/A	N/A	FC IHC (F)	RUO
17-9957	Anti-Human HLA-G APC	633	660	FC	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintenance of transplanted organs.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 87G antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 87G antibody has been pre-titrated and tested by flow cytometric analysis of U937 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.

References

Carosella ED, Favier B, Rouas-Freiss N, Moreau P, Lemaoult J. Beyond the increasing complexity of the immunomodulatory HLA-G molecule. Blood. 2008 May 15;111(10):4862-70. Epub 2008 Mar 11. Review.

Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE. Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol. 2003 Aug 1;171(3):1376-84.(87G, FC, IHfrozen, PubMed)

Rouas-Freiss N, Moreau P, Menier C, Carosella ED. HLA-G in cancer: a way to turn off the immune system. Semin Cancer Biol. 2003 Oct;13(5):325-36. Review.

Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED. HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.

Onno M, Le Friec G, Pangault C, Amiot L, Guilloux V, Drénou B, Caulet-Maugendre S, André P, Fauchet R. Modulation of HLA-G antigens expression in myelomonocytic cells. Hum Immunol. 2000 Nov;61(11):1086-94. (87G, FC, PubMed)

Yang Y, Chu W, Geraghty DE, Hunt, JS Expression of HLA-G in human mononuclear phagocytes and selective induction by IFN-gamma J. Immunol., 1996 June;156:4224-4231.

Odum N, Ledbetter JA, Martin P, Geraghty D, Tsu T, Hansen JA, Gladstone P. Homotypic aggregation of human cell lines by HLA class II-, class Ia- and HLA-G-specific monoclonal antibodies. Eur J Immunol. 1991 Sep;21(9):2121-31,(87G FC, PubMed)

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.