Contents: Allophycocyanin (APC) anti-human TNF-α
Catalog Number: 17-7349
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAb11
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD4 (RPA-T4) and intracellularly stained with PE anti-human TNF-a (Mab11).
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7349 |
Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
N/A |
N/A |
IC Flow
|
| 13-7349 |
Biotin anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
N/A |
N/A |
ELISA det
ELISPOT det
|
| 11-7349 |
FITC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
518 |
IC Flow
|
| 12-7349 |
PE anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
575 |
IC Flow
|
| 17-7349 |
APC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
633 |
660 |
IC Flow
|
| 25-7349 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
760 |
IC Flow
|
| 51-7349 |
Alexa Fluor® 647 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
633 |
668 |
IC Flow
|
| 53-7349 |
Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
519 |
IC Flow
|
| 57-7349 |
Pacific Blue® anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
402 |
421 |
IC Flow
|
| 45-7349 |
PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) |
488 |
690 |
IC Flow
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4
+ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MAb11 has been reported for use in intracellular flow cytometric analysis.
Applications Tested
The MAb11 antibody has been tested for intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells.
References
Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes."
Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors."
Mol Immunol 28(1-2): 79-86.
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