Contents: Allophycocyanin (APC) anti-human CCL2 (MCP-1, MCP1, MCAF)
Catalog Number: 17-7099
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 5D3-F7
Isotype: Mouse IgG1, κ
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*International customers: Please contact your distributor for region specific pricing.
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Staining of LPS-treated human peripheral blood mononuclear cells with FITC anti-human CD14 (61D3) (cat. 11-0149) and 0.03 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (left) or APC anti-human CCL2/MCP-1 (5D3-F7) (right). Cells in the monocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7099 |
Affinity Purified anti-human CCL2 (MCP-1, MCP1, MCAF) |
N/A |
N/A |
ELISA cap
IC Flow
IHC(Paraffin)
IP
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| 16-7099 |
Functional Grade* Purified anti-human CCL2 (MCP-1, MCP1, MCAF) |
N/A |
N/A |
ELISA cap
NU
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| 17-7099 |
Allophycocyanin (APC) anti-human CCL2 (MCP-1, MCP1, MCAF) |
633 |
660 |
IC Flow
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This 5D3-F7 antibody is offered in 2 formats:
- μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.
References
Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257.
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17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control
