Staining of LPS-treated human peripheral blood mononuclear cells with FITC anti-human CD14 (61D3) (cat. 11-0149) and 0.03 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (left) or APC anti-human CCL2/MCP-1 (5D3-F7) (right). Cells in the monocyte gate were used for analysis.
Contents: Allophycocyanin (APC) anti-human CCL2 (MCP-1, MCP1, MCAF) Catalog Number: 17-7099 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: 5D3-F7 Host/Isotype: Mouse IgG1, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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17-7099-81
50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This 5D3-F7 antibody is offered in 2 formats:
- μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.
References
Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257. (5D3-F7, ELISA, WB, IHC frozen),
Karrer S, Bosserhoff AK, et al. 2005. The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis. J Invest Dermatol. 124(1):92-8. (5D3-F7, IHC paraffin, PubMed)
Piemonti L, Leone BE, et al. 2002. Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation. Diabetes. 51(1):55-65. (5D3-F7, IHC paraffin, PubMed)
