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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications TestedThis 5D3-F7 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization. References
Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257. Related ProductsCat. 17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control |
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