Product Information

Contents: Allophycocyanin (APC) anti-human interleukin-4 (IL-4)
Catalog Number: 17-7048
Sizes: 25 tests, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT SENSITIVE MATERIAL.
Clone: MP4-25D2
Isotype: Rat IgG1, κ

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-4 (MP4-25D2). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-4.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7048	Affinity Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	IC Flow
16-7048	Functional Grade* Purified anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	NU
13-7048	Biotin anti-human IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA det ELISPOT det
11-7048	FITC anti-human IL-4 (Interleukin-4, IL4)	488	518	IC Flow
12-7048	PE anti-human IL-4 (Interleukin-4, IL4)	488	575	IC Flow
17-7048	APC anti-human IL-4 (Interleukin-4, IL4)	633	660	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MP4-25D2 has been reported for use in intracellular flow cytometric analysis.

Applications Tested

This MP4-25D2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human PBMCs. This can be used at less than or equal to 0.03 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ramanathan, L., R. Ingram, et al. (1993). Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies. Biochemistry 32(14): 3549-56.
Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.

Related Products

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