Contents: Allophycocyanin (APC) anti-human interleukin-4 (IL-4)
Catalog Number: 17-7048
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT SENSITIVE MATERIAL.
Clone: MP4-25D2
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-4 (MP4-25D2). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-4.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7048 |
FITC anti-human IL-4 (Interleukin-4, IL4) |
488 |
518 |
IC Flow
|
| 12-7048 |
PE anti-human IL-4 (Interleukin-4, IL4) |
488 |
575 |
IC Flow
|
| 13-7048 |
Biotin anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
ELISA det
ELISPOT det
|
| 14-7048 |
Affinity Purified anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
IC Flow
|
| 16-7048 |
Functional Grade* Purified anti-human IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
NU
|
| 17-7048 |
APC anti-human IL-4 (Interleukin-4, IL4) |
633 |
660 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MP4-25D2 has been reported for use in intracellular flow cytometric analysis.
Applications Tested
This MP4-25D2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human PBMCs. This can be used at less than or equal to 0.03 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ramanathan, L., R. Ingram, et al. (1993). Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies. Biochemistry 32(14): 3549-56.
Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.
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