Contents: Allophycocyanin (APC) anti-mouse interleukin-4 (IL-4)
Catalog Number: 17-7041
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT SENSITIVE MATERIAL.
Clone: 11B11
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 12-7041 |
PE anti-mouse IL-4 (Interleukin-4, IL4) |
488 |
575 |
IC Flow
|
| 14-7041 |
Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
ELISA cap
ELISPOT cap
|
| 16-7041 |
Functional Grade* Purified anti-mouse IL-4 (Interleukin-4, IL4) |
N/A |
N/A |
NU
|
| 17-7041 |
APC anti-mouse IL-4 (Interleukin-4, IL4) |
633 |
660 |
IC Flow
|
| 51-7041 |
Alexa Fluor® 647 anti-mouse IL-4 (Interleukin-4, IL4) |
633 |
668 |
IC Flow
|
| 53-7041 |
Alexa Fluor® 488 anti-mouse IL-4 (Interleukin-4, IL4) |
488 |
519 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 11B11 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-4 bioactivity, IHC, and intracellular staining for flow cytometric analysis.
Applications Tested
The 11B11 antibody has been tested for intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells.
References
Ohara, J. and W. E. Paul. 1985. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 315: 333-336.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.
Related Products
Cat.
24-5093 Polyclonal anti-mouse IgD antiserum (activating) (clone Polyclonal)
