Product Information

Contents: Allophycocyanin (APC) anti-human interleukin-2 (IL-2)
Catalog Number: 17-7029
Sizes: 25 tests, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MQ1-17H12
Isotype: Rat IgG2a, κ

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. (Left) The cells were surface stained with PE anti-human CD3 (UCHT1) and intracellularly stained with FITC anti-human IL-2 (MQ1-17H12). (Right) The cells were surface stained with FITC anti-human CD3 and intracellularly stained with PE anti-human IL-2.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7029	FITC anti-human IL-2 (Interleukin-2, IL2)	488	518	IC Flow
12-7029	PE anti-human IL-2 (Interleukin-2, IL2)	488	575	IC Flow
14-7029	Affinity Purified anti-human IL-2 (Interleukin-2, IL2)	N/A	N/A	ELISA cap IC Flow
17-7029	APC anti-human IL-2 (Interleukin-2, IL2)	633	660	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MQ1-17H12 has been reported for use in staining of intracellular IL-2.

Applications Tested

This MQ1-17H12 antibody has been tested intracellular staining and flow cytometric analysis of activated human PBMC. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Andersson, J., J. Abrams, et al. 1994. "Concomitant in vivo production of 19 different cytokines in human tonsils." Immunology 83: 16-24.
Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.

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