Also known as: RORgamma, RORg, RORg(t), Retinoid-Related Orphan Receptor gamma
RUO: For Research Use Only
Surface staining of mouse thymocytes with Anti-Mouse CD4 FITC (cat. 11-0041) and Anti-Mouse CD8a PE (cat. 12-0081) (left), followed by intracellular staining with Anti-Human/Mouse RORγ(t) APC (right), using Foxp3 Staining Buffers (cat. 00-5523). Cells in the CD4+CD8+ (purple histogram) or CD4+CD8- (blue histogram) gate were used for analysis.
Additional data demonstrating staining of RORγ(t) with the AFKJS-9 monoclonal antibody in mouse and human Th17-polarized T cells, may be viewed on the Th17 cells webpage.
Contents: Anti-Human/Mouse ROR gamma (t) APC Catalog Number: 17-6988 Concentration: 0.2 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: AFKJS-9 Host/Isotype: Rat IgG2a
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17-6988-80
25 ug
17-6988-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The AJKJS-9 monoclonal antibody reacts with the mouse RORγ(t) protein. RORγ is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORα and RORβ. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORγt, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ “double-positive” thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
The RORγt isoform differs from RORγ by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the RORγ and RORγt isoforms.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This AFKJS-9 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using Foxp3 Staining Buffers. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Use of the eBioscience Foxp3 Staining Buffer Set (cat. 00-5523) is recommended when using this product for intracellular staining followed by flow cytometry.
References
Schmutz S, Bosco N, Chappaz S, Boyman O, Acha-Orbea H, Ceredig R, Rolink AG, Finke D. Cutting edge: IL-7 regulates the peripheral pool of adult RORgamma+ lymphoid tissue inducer cells. J Immunol. 2009 Aug 15;183(4):2217-21 (AFKJS-9, IC flow, PubMed)
Poffenberger MC, Straka N, El Warry N, Fang D, Shanina I, Horwitz MS. Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis. PLoS One. 2009 Jul 9;4(7):e6207. (AFKJS-9, IC flow, PubMed)
Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ, Littman DR. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells.
Cell. 2006 Sep 22;126(6):1121-33.
Sun Z, Unutmaz D, Zou YR, Sunshine MJ, Pierani A, Brenner-Morton S, Mebius RE, Littman DR. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000 Jun 30;288(5475):2369-73.
He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells.
Immunity. 1998 Dec;9(6):797-806.
Medvedev A, Yan ZH, Hirose T, Gigučre V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996 Nov 28; 181(1-2):199-206.
