Product Information

Contents: Allophycocyanin (APC) anti-human B7-H1 (CD274, B7H1, PDL-1, PD-L1)
Catalog Number: 17-5983
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MIH1
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (open histogram) or APC anti-human B7-H1 (MIH1) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5983	Affinity Purified anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC IH/F WB
16-5983	Functional Grade* Purified anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC
13-5983	Biotin anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC
12-5983	PE anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	575	FC
17-5983	Allophycocyanin (APC) anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	633	660	FC
25-5983	Phycoerythrin-Cy7 (PE-Cy7) anti-human B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MIH1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.

Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.

Saudemont, A., Jouy, N., Hetuin, D., and Quesnel, B. 2005. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 105:2428-2435.

Ohigashi Y, Sho M, Yamada Y, Tsurui Y, Hamada K, Ikeda N, Mizuno T, Yoriki R, Kashizuka H, Yane K, Tsushima F, Otsuki N, Yagita H, Azuma M, Nakajima Y. Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer. Clin Cancer Res. 2005 Apr 15;11(8):2947-53. (MIH1, IH/F, PubMed)

Chen Y, Zhang J, Li J, Zou L, Zhao T, Tang Y, Wu Y. Expression of B7-H1 in inflammatory renal tubular epithelial cells. Nephron Exp Nephrol. 2006;102(3-4):e81-92. (MIH1, IH/F, FA, PubMed)

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