Contents: Allophycocyanin (APC) anti-mouse GITR (TNFRSF18)
Catalog Number: 17-5874
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DTA-1
Isotype: Rat IgG2b
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 splenocytes with FITC anti-mouse CD25 (PC61.5) (cat. 11-0251) and .03 μg of APC Rat IgG2a Iso Cntrl (cat. 17-4321) (left) or .03 μg of APC DTA-1 (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5874 |
Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR) |
488 |
518 |
FC
|
| 12-5874 |
Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR) |
488 |
575 |
FC
|
| 13-5874 |
Biotin anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FC
|
| 14-5874 |
Affinity Purified anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FA
FC
IP
|
| 16-5874 |
Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FA
FC
|
| 17-5874 |
Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR) |
633 |
660 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4
+CD25
+ T regulatory cells (Treg) and by CD25
+ CD4
+ CD8
- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This DTA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.
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Mouse Cellular Antigens Chart