Contents: Allophycocyanin (APC) anti-mouse DO11.10 TCR
Catalog Number: 17-5808
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: KJ1-26 (KJ126, KJI-26, KJI26)
Isotype: Mouse IgG2a
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
Staining of the DO11.10 T cell hybridoma with 0.125 μg of APC Mouse IgG2a, K Iso Cntrl (cat. 17-4724) (open histogram) or 0.125 μg of APC anti-mouse DO11.10 TCR (KJ1-26) (colored histogram). Total viable cells were used for analysis.
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The KJ1-26 anti-idiotypic monoclonal antibody binds the
T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell
hybridomas, but not on any other T cell hybridomas tested.The TCR expressed
on DO11.10 recognizes an ovalbumin peptide in the context of I-A
d major
histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to
be instrumental in describing many of the intricate details of T cell
immunity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of the DO11.10 T cell hybridoma. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Haskins, K., R. Kubo, J. White, M. Pigeon, J. Kappler, and P. Marrack.
1983. The major histocompatibility complex-restricted antigen receptor on
T cells. I. Isolation with a monoclonal antibody. J. Exp. Med. 157: 1149-
69.
Marrack, P., R. Shimonkevitz, C. Hannum, K. Haskins, and J. Kappler.
1983. The major histocompatibility complex-restricted antigen receptor on
T cells. IV. An anti-idiotypic antibody predicts both antigen and Ispecificity.
J. Exp. Med. 158: 1635-46.
Murphy, K. M., A. B. Heimberger, and D. Y. Loh. 1990. Induction by
antigen of intrathymic apoptosis of CD4+ CD8+ TCR lo thymocytes in vivo.
Science. 250: 1720-3.
Related Products
Cat.
17-4724 Allophycocyanin (APC) Mouse IgG2a, K Isotype Control
Mouse Cellular Antigens Chart