Product Information

Contents: Allophycocyanin-conjugated Streptavidin
(SAV-APC)
Catalog Number: 17-4317
Sizes: 100 ug
Formulation: 10mM PBS/500 mM NaCl,
0.09% NaN₃, pH 7.2
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)	488	518	FC
12-4317	Streptavidin-PE (Phycoerythrin)	488	575	FC
15-4317	Streptavidin-PE-Cy5 (Phycoerythrin-Cy5)	488	670	FC
17-4317	Streptavidin Allophycocyanin (SA-APC)	633	660	FC
19-4317	Streptavidin-Cy5	633	670	FC
25-4317	Streptavidin-PE-Cy7 (Phycoerythrin-Cy7)	488	760	FC
35-4317	Streptavidin-PE-Cy5.5	488	690	FC
45-4317	Streptavidin-PerCP-Cy5.5 (PE-Cy5.5 replacement)	488	690	FC
93-4317	eFluor^TM 605^NC Streptavidin conjugate	350-500	605	FC
94-4317	eFluor^TM 625^NC Streptavidin conjugate	350-500	625	FC
95-4317	eFluor^TM 650^NC Streptavidin	350-500	650	FC
47-4317	Allophycocyanin-eFluor^TM 780 Streptavidin (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-4317	eFluor^TM 450 Streptavidin (Pacific Blue® replacement)	405	450	FC
49-4317	eFluor^TM 710 Streptavidin (APC-Cy5.5 replacement)	633	710	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. SAV-APC is excited at 610-640 nm and emits its fluorescence at ~660 nm.

Applications Tested

Streptavidin-APC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. This can be used at less than or equal to 0.06 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.