Product Information

Contents: Allophycocyanin (APC) anti-human PD-1 (CD279, PD1)
Catalog Number: 17-2799
Sizes: 25 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioJ105 (J105)
Isotype: Mouse IgG1, κ

Staining of 3-day PHA activated PBMCs with FITC anti-human PD-1 (MIH4) (cat. 11-9969) and 1.0 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (left) or APC anti-human PD-1 (eBioJ105) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2799	Affinity Purified anti-human PD-1 (CD279, PD1)	N/A	N/A	FC IH/F IP
13-2799	Biotin anti-human PD-1 (CD279, PD1)	N/A	N/A	FC
12-2799	Phycoerythrin (PE) anti-human PD-1 (CD279, PD1)	488	575	FC
17-2799	Allophycocyanin (APC) anti-human PD-1 (CD279, PD1)	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.
Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated PBMCs. This can be used at 20 μl (1 μg) per million cells in a 100 μl total staining volume.

References

Iwai Y, Okazaki T, Nishimura H, Kawasaki A, Yagita H, Honjo T. Microanatomical localization of PD-1 in human tonsils. Immunol Lett. 2002 Oct 1;83(3):215-20. PubMed

Related Products

Cat. 17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control
Cat. 17-9969 Allophycocyanin (APC) anti-human PD-1 (CD279, PD1) (clone MIH4)