Staining of C57BL/6 thymocytes with 0.125 µg of Mouse IgG2a κ Isotype Control APC (cat. 17-4724) (blue histogram) or 0.125 µg of Anti-Mouse CD199 (CCR9) APC (purple histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD199 (CCR9) APC Catalog Number: 17-1991 Concentration: 0.2 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBioCW-1.2 (CW-1.2) Host/Isotype: Mouse IgG2a
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Cat. No.
Size
Price
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17-1991-80
25 ug
17-1991-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naļve CD8+ T cells, but not on naļve CD4+ T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y. Coordination between CCR7- and CCR9-mediated chemokine signals in pre-vascular fetal thymus colonization. Blood. 2006 Jun 29.
Wurbel MA, Malissen B, Campbell JJ. Complex regulation of CCR9 at multiple discrete stages of T cell development. Eur J Immunol. 2006 Jan;36(1):73-81. (CW-1.2, FC, Development of mAb, PubMed)
Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999 May 15;162(10):5671-5.
