Product Information

Contents: Allophycocyanin (APC) anti-human CD193 (CCR3, CKR3)
Catalog Number: 17-1939
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio5E8-G9-B4 (5E8-G9-B4)
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with PE anti-human CD16 (CB16) (cat. 12-0168) and APC Mouse IgG2b Iso Cntrl (cat. 17-4732) (left) or APC anti-human CD193 (5E8-G9-B4) (right). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1939	Affinity Purified anti-human CCR3 (CD193, CKR3)	N/A	N/A	FC
12-1939	Phycoerythrin (PE) anti-human CCR3 (CD193, CKR3)	488	575	FC
17-1939	Allophycocyanin (APC) anti-human CCR3 (CD193, CKR3)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Choe H, Farzan M, Sun Y, Sullivan N, Rollins B, Ponath PD, Wu L, Mackay CR, LaRosa G, Newman W, Gerard N, Gerard C, Sodroski J. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996 Jun 28;85(7):1135-48.

Heath H, Qin S, Rao P, Wu L, LaRosa G, Kassam N, Ponath PD, Mackay CR. Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody. J Clin Invest. 1997 Jan 15;99(2):178-84.

Forssmann U, Uguccioni M, Loetscher P, Dahinden CA, Langen H, Thelen M, Baggiolini M. Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes. J Exp Med. 1997 Jun 16;185(12):2171-6.

Sallusto F, Mackay CR, Lanzavecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science. 1997 Sep 26;277(5334):2005-7.

Related Products

Cat. 17-4732 Allophycocyanin (APC) Mouse IgG2b Isotype Control