Staining of normal human peripheral blood cells with Anti-Human CD16 PE (cat. 12-0168) and Mouse IgG2b κ Isotype Control APC (cat. 17-4732) (left) or Anti-Human CD193 (CCR3) APC (right). Cells in the large scatter gate were used for analysis.
Contents: Anti-Human CD193 (CCR3) APC Catalog Number: 17-1939 Concentration: 20 μl (0.25 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBio5E8-G9-B4 (5E8-G9-B4) Host/Isotype: Mouse IgG2b, κ
Product Options
Cat. No.
Size
Price
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17-1939-71
25 tests
17-1939-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Choe H, Farzan M, Sun Y, Sullivan N, Rollins B, Ponath PD, Wu L, Mackay CR, LaRosa G, Newman W, Gerard N, Gerard C, Sodroski J.
The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates.
Cell. 1996 Jun 28;85(7):1135-48.
Heath H, Qin S, Rao P, Wu L, LaRosa G, Kassam N, Ponath PD, Mackay CR.
Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody.
J Clin Invest. 1997 Jan 15;99(2):178-84.
Forssmann U, Uguccioni M, Loetscher P, Dahinden CA, Langen H, Thelen M, Baggiolini M.
Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes.
J Exp Med. 1997 Jun 16;185(12):2171-6.
Sallusto F, Mackay CR, Lanzavecchia A.
Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells.
Science. 1997 Sep 26;277(5334):2005-7.
