Staining of C57Bl/6 splenocytes with FITC anti-mouse CD4 (GK1.5) (cat. 11-0041) and 0.25 μg of APC Armenian Hamster IgG Iso Cntrl (cat. 17-4888) (left) or 0.25 μg of APC anti-mouse CD183 (CXCR3-173) (right). Total viable cells were used for analysis.
Contents: Allophycocyanin (APC) anti-mouse CD183 (CXCR3) Catalog Number: 17-1831 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: CXCR3-173 Host/Isotype: Armenian Hamster IgG Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Cat. No.
Size
Price
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17-1831-80
25 ug
17-1831-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The monoclonal antibody CXCR3-173 recognizes mouse CD183 also known as CXCR3. CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL19 (mig), CXCL10 (IP-10)and CXCL11 (ITAC). CD183 as been shown to play a role in CD4 T cell responses to grafts. CXCR3 knockout mice have compromised allograft rejection responses. Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarized cells.
The antibody CXCR3-173 has been shown to affect chemotaxis in response to ligand. The presence of ligand eliminates staining with the antibody. In vivo addition of the antibody delays cardiac and pancreatic islet allograft rejection.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This CXCR3-173 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This CXCR3-173 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Uppaluri R, Sheehan KC, Wang L, Bui JD, Brotman JJ, Lu B, Gerard C, Hancock WW, Schreiber RD. Prolongation of Cardiac and Islet Allograft Survival by a Blocking Hamster Anti-Mouse CXCR3 Monoclonal Antibody.
Transplantation. 2008 Jul 15;86(1):137-147. (CXCR3, FA, FC PubMed)
Morimoto Y, Bian Y, Gao P, Yashiro-Ohtani Y, Zhou XY, Ono S, Nakahara H, Kogo M, Hamaoka T, Fujiwara H. Induction of surface CCR4 and its functionality in mouse Th2 cells is regulated differently during Th2 development.
J Leukoc Biol. 2005 Sep;78(3):753-61.
