Product Information

Contents: Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L)
Catalog Number: 17-1548
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 24-31
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Staining of unstimulated PBMC's with 0.5 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (open histogram) or APC anti-human CD154 (24-31) (colored histogram) on left. Staining of 6 hour PMA/ionomycin stimulated PBMC's with 0.5 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (open histogram) or APC anti-human CD154 (24-31) (colored histogram) on right. Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1548	FITC anti-human CD154 (CD40 Ligand, CD40L)	488	518	FC
12-1548	PE anti-human CD154 (CD40 Ligand, CD40L)	488	575	FC
14-1548	Affinity Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
16-1548	Functional Grade* Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
17-1548	Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L)	633	660	FC
27-1548	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD154 (CD40 Ligand, CD40L)	633	779	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the Cynomogus monkey CD154.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 24-31 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of activated human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Brams, P., A. Black, et al. 2001. A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol 1(2): 277-94.

Related Products

Cat. 17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control