Product Information

Contents: Allophycocyanin (APC) anti-human CD134 (OX40, OX-40)
Catalog Number: 17-1347
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ACT35 (ACT-35)
Isotype: Mouse IgG1

Surface staining of PHA stimulated human PBMC with anti-human CD134 (ACT35 ) APC. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1347	Affinity Purified anti-human CD134 (OX40, OX-40)	N/A	N/A	FC
11-1347	Fluorescein isothiocyanate (FITC) anti-human CD134 (OX40, OX-40)	488	518	FC
12-1347	Phycoerythrin (PE) anti-human CD134 (OX40, OX-40)	488	575	FC
17-1347	Allophycocyanin (APC) anti-human CD134 (OX40, OX-40)	633	660	FC

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Description

The ACT35 monoclonal antibody reacts with human CD134, also known as OX-40. A member of the TNF receptor superfamily, CD134/OX-40 is a 50 kDa type I membrane glycoprotein expressed by activated T lymphocytes. The interaction of CD134 with OX-40L has been implicated in T cell-dependent humoral response, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool and regulation of tolerance in the CD4⁺ T cell compartment.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ACT35 (ACT-35) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This ACT35 (ACT-35) antibody has been pre-titrated and tested by flow cytometric analysis of 3-day PHA activated human PBMC. This can be used at 5 μl (1 μg) per million cells in a 100 μl total staining volume.

References

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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