Contents: Allophycocyanin (APC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)
Catalog Number: 17-1231
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 5B11
Isotype: Rat IgG2a, κ
| Prices for This Product* |
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Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of BALB/c bone marrow cells with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 0.25 μg of APC Rat IgG2a Iso Cntrl (cat. 17-4321) (left) or 0.25 μg of APC anti-mouse CD123 (5B11) (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1231 |
Affinity Purified anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) |
N/A |
N/A |
FC
|
| 13-1231 |
Biotin anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) |
N/A |
N/A |
FC
|
| 11-1231 |
Fluorescein isothiocyanate (FITC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) |
488 |
518 |
FC
|
| 12-1231 |
PE anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) |
488 |
575 |
FC
|
| 17-1231 |
Allophycocyanin (APC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra) |
633 |
660 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 5B11 monoclonal antibody reacts with mouse CD123, the α chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common β chain) or AIC2A (IL-3β) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the β chain for this function.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 5B11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ichihara, M., T. Hara, et al. (1995). "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene."
Embo J 14(5): 939-50.
Mueller, D. L., Z. M. Chen, et al. (1994). "Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth."
J Immunol 153(7): 3014-27.
Related Products
Cat.
17-4321 APC Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart