Product Information

Contents: Allophycocyanin (APC) anti-mouse CD117 (c-Kit, cKit)
Catalog Number: 17-1171
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2B8
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of APC Rat IgG2b Isotype Control (cat. 17-4031) (left) or 0.06 μg of APC anti-mouse CD117 (2B8) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1171	Affinity Purified anti-mouse CD117 (c-Kit, cKit)	N/A	N/A	FC IP
13-1171	Biotin anti-mouse CD117 (c-Kit, cKit)	N/A	N/A	FC
11-1171	FITC anti-mouse CD117 (c-Kit, cKit)	488	518	FC
12-1171	PE anti-mouse CD117 (c-Kit, cKit)	488	575	FC
15-1171	PE-Cy5 anti-mouse CD117 (c-Kit, cKit)	488	670	FC
17-1171	APC anti-mouse CD117 (c-Kit, cKit)	633	660	FC
19-1171	Cy5 anti-mouse CD117 (c-Kit, cKit)	633	670	FC
25-1171	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD117 (c-Kit, cKit)	488	760	FC
35-1171	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD117 (c-Kit, cKit)	488	690	FC
47-1171	Allophycocyanin-eFluor^TM 780 anti-mouse CD117 (c-Kit, cKit) (APC-Alexa Fluor® 750 replacement)	633	780	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2B8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ikuta K, Weissman IL. 1992. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci USA. 89(4): 1502-6.

Related Products

Cat. 17-4031 Allophycocyanin (APC) Rat IgG2b Isotype Control