Contents: Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)
Catalog Number: 17-0959
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64
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Staining of normal human peripheral blood cells with 0.25 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (blue histogram) or APC anti-human CD95 (DX2) (purple histogram). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.
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Description
The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of
EOS9.1, another antibody specific for human CD95.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This DX2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
References
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
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Cat.
17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart