Product Information

Contents: Allophycocyanin (APC) anti-human CD93 (C1qRp)
Catalog Number: 17-0939
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: R139
Isotype: Mouse IgG2b

Staining of normal human peripheral blood cells with 0.125 μg of APC Mouse IgG2b Iso Cntrl (cat. 17-4732) (open histogram) or APC anti-human CD93 (R139) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0939	Affinity Purified anti-human CD93 (C1qRp)	N/A	N/A	ELISA cap FC IP WB
17-0939	Allophycocyanin (APC) anti-human CD93 (C1qRp)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody R139 recognizes human CD93, also known as C1qRp. The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A. CD93 is predicted to play a role in the clearance of apoptotic cells. Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells. Expression on DC’s is downregulated upon maturation. Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors.
The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis.
CD93 can be shed from the cell surface. This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA. The epitope for R139 resides in the EGF domains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This R139 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This R139 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Bohlson SS, Silva R, Fonseca MI, Tenner AJ. CD93 is rapidly shed from the surface of human myeloid cells and the soluble form is detected in human plasma. J Immunol. 2005 Jul 15;175(2):1239-47. (R139 R3 ELISA, WB PubMed)

Danet GH, Luongo JL, Butler G, Lu MM, Tenner AJ, Simon MC, Bonnet DA. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential. Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10441-5.(R139, FC PubMed)

Maruyama H, Galvan M, Waffarn F, Tenner AJ. Human cord blood leukocyte innate immune responses to defense collagens. Pediatr Res. 2003 Nov;54(5):724-31.(R139, FA, PubMed).

Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998 Feb 15;160(4):1929-35. (R139, FC, PubMed)

Related Products

Cat. 17-4732 Allophycocyanin (APC) Mouse IgG2b Isotype Control
Cat. 17-5892 APC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) (clone AA4.1)