Contents: Allophycocyanin (APC) anti-rat MHC Class II
Catalog Number: 17-0920
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS19
Isotype: Mouse IgG1
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Description
The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HIS19 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Stet, R.J.M., J. Rozing, et.al.. 1985. HIS19: a monoclonal antibody recognizing a class II polymorphic determinant only absent on RT1n class II antigens. Transplant. Proc. 17: 182901831.
Uyama, T., J. B. Winter, et al. 1993. Replacement of dendritic cells in the airways of rat lung allografts. Am Rev Respir Dis 148(3): 760-7.
Stet, R. J., C. Thomas, et al. 1985. Graft versus host disease in the rat: Ia expression on target tissues. Adv Exp Med Biol 186: 545-54.
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