Allophycocyanin (APC) anti-mouse CD90.2 (Thy-1.2, Thy1.2)

RUO: For Research Use Only

Contents: Allophycocyanin (APC) anti-mouse CD90.2 (Thy-1.2, Thy1.2)
Catalog Number: 17-0902
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: 53-2.1
Host/Isotype: Rat IgG2a, κ
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
17-0902-81	50 ug	To view relevant pricing, please select your country and resume browsing.
17-0902-82	100 ug
17-0902-83	200 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-0903	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (30-H12)
12-0903	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2) (30-H12)
13-0903	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2) (30-H12)
14-0903	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (30-H12)
16-0903	Functional Grade Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (30-H12)
17-4321	APC Rat IgG2a Isotype Control

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0902	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	518	FC	RUO
12-0902	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	575	FC	RUO
13-0902	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC	RUO
14-0902	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC IH/F IP	RUO
15-0902	PE-Cy5 anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	670	FC	RUO
17-0902	APC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	633	660	FC	RUO
25-0902	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	760	FC	RUO
47-0902	Allophycocyanin-eFluor® 780 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (APC-Alexa Fluor® 750 replacement)	633	780	FC	RUO
48-0902	eFluor® 450 anti-mouse CD90.2 (Thy-1.2, Thy1.2) (Pacific Blue® replacement)	405	450	FC	RUO
95-0902	eFluor® 650NC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	350-500	650	FC	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.