Surface staining of rat splenocytes with anti-rat/Mouse CD90.1 (HIS51) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Allophycocyanin (APC) anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1) Catalog Number: 17-0900 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: HIS51 Host/Isotype: Mouse IgG2a, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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17-0900-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kroese, F. G., N. K. de Boer, et al. 1995. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell Immunol 162(2): 185-93. Hosseinzadeh, H. and I. Goldschneider 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol 150(5): 1670-9. Ishizu, A., H. Ishikura, et al. 1995. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol 7(12): 1939-47. Hermans, M. H. and D. Opstelten 1991. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem 39(12): 1627-34.
