Product Information
Contents: Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA)
Catalog Number: 17-0691
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: H1.2F3
Isotype: Armenian Hamster IgG
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Staining of 1-day ConA activated BALB/c splenocytes with 0.125 μg of APC Armenian Hamster IgG Iso Cntrl (cat. 17-4888) (blue histogram) or 0.125 μg of APC anti-mouse CD69 ((H1.2F3 ) (purple histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0691 |
Affinity Purified anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0691 |
Functional Grade* Purified anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FA
FC
|
| 13-0691 |
Biotin anti-mouse CD69 (Very Early Activation Antigen, VEA) |
N/A |
N/A |
FC
|
| 11-0691 |
FITC anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
518 |
FC
|
| 12-0691 |
PE anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
575 |
FC
|
| 15-0691 |
PE-Cy5 anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
670 |
FC
|
| 17-0691 |
Allophycocyanin (APC) anti-mouse CD69 (Very Early Activation Antigen, VEA) |
633 |
660 |
FC
|
| 25-0691 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD69 (Very Early Activation Antigen, VEA) |
488 |
760 |
FC
|
| 45-0691 |
PerCP-Cy5.5 anti-mouse CD69 (Very Early Activation Antigen, VEA) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
| 93-0691 |
eFluorTM 605NC anti-mouse CD69 (Very Early Activation Antigen, VEA) |
350-500 |
605 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This H1.2F3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Yokoyama, W. M., F. Koning, et al. (1988). "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation."
J Immunol 141(2): 369-76.
Related Products
Cat. 17-0699 Allophycocyanin (APC) anti-human CD69 (Very Early Activation Antigen, VEA) (clone FN50)Cat. 17-4888 Allophycocyanin (APC) Armenian Hamster IgG Isotype Control (clone eBio299Arm)
