Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (cat. 11-0037) and Mouse IgG1 κ Isotype Control APC (cat. 17-4714) (left) or Anti-Human CD56 (NCAM) APC (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD56 (NCAM) APC Catalog Number: 17-0567 Concentration: 5 µl (0.125 µg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: CMSSB Host/Isotype: Mouse IgG1, κ
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17-0567-41
25 tests
17-0567-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
This CMSSB monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells. Staining with CMSSB does not block binding of MEM188 or CB56.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This CMSSB antibody has been reported for use in flow cytometric analysis.
Applications Tested
This CMSSB antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Hayakawa Y, Huntington ND, Nutt SL, Smyth MJ. 2006 Functional subsets of mouse natural killer cells. Immunol Rev. 214:47-55.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.
