Contents: Allophycocyanin (APC) anti-human CD45 (LCA)
Catalog Number: 17-0459
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816
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Surface staining of normal human peripheral blood cells with anti-human CD45 (HI30) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
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Description
The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI30 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart