Product Information

Contents: Allophycocyanin (APC) anti-human CD45RA
Catalog Number: 17-0458
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Staining of normal human lysed whole blood with APC Mouse IgG2b Iso Cntrl (cat. 17-4732) (open histogram) or APC anti-human CD45RA (HI100) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA (PE-Cy5.5 replacement)	488	690	FC
93-0458	eFluor^TM 605^NC anti-human CD45RA	350-500	605	FC
48-0458	eFluor^TM 450 anti-human CD45RA (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (.008 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 17-4732  Allophycocyanin (APC) Mouse IgG2b Isotype Control
Cat. 11-9979  FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979  PE anti-human CD45RA (clone JS-83)
Cat. 13-9979  Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979  Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979  PE-Cy5 anti-human CD45RA (clone JS-83)