APC anti-human CD45RA antibody: allophycocyanin conjugate

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Contents: Allophycocyanin (APC) anti-human CD45RA
Catalog Number: 17-0458
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
17-0458-71	25 tests	$120
17-0458-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human lysed whole blood with APC Mouse IgG2b Iso Cntrl (cat. 17-4732) (open histogram) or APC anti-human CD45RA (HI100) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA	488	690	FC
57-0458	Pacific Blue® anti-human CD45RA	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (.008 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 17-4732    Allophycocyanin (APC) Mouse IgG2b Isotype Control
Cat. 11-9979    FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979    PE anti-human CD45RA (clone JS-83)
Cat. 13-9979    Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979    Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979    PE-Cy5 anti-human CD45RA (clone JS-83)