Contents: Allophycocyanin (APC) anti-human CD45RO
Catalog Number: 17-0457
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHL1
Isotype: Mouse IgG2a, κ
HLDA No.: IV N31
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Staining of normal human peripheral blood cells with APC Mouse IgG2a, K Iso Cntrl (cat. 17-4724) (open histogram) or APC anti-human CD45RO (UCHL1) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This UCHL1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.03 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
17-4724 Allophycocyanin (APC) Mouse IgG2a, K Isotype Control
Human Cellular Antigens Chart