Contents: Allophycocyanin (APC) anti-mouse CD45RB
Catalog Number: 17-0455
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C363.16A
Isotype: Rat IgG2a, κ
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Staining of C57Bl/6 splenocytes with 0.125 μg of APC Rat IgG2a Iso Cntrl (cat. 17-4321) (open histogram) or 0.125 μg of APC anti-mouse CD45RB (C363.16A) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This C363.16A antibody has been reported for use in flow cytometric analysis.
Applications Tested
This C363.16A antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Bottomly, K., M. Luqman, et al. (1989). "A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines." Eur J Immunol 19(4): 617-23.
Johnson, P., L. Greenbaum, et al. (1989). "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." J Exp Med 169(3): 1179-84.
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