Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg APC anti-mouse/human CD45R (B220)(RA3-6B2) (colored histogram). Total viable cells were used for analysis.
Contents: Allophycocyanin (APC) anti-mouse/human CD45R (B220) Catalog Number: 17-0452 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: RA3-6B2 Host/Isotype: Rat IgG2a, κ HLDA Workshop: N/A Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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17-0452-81
50 ug
17-0452-82
100 ug
17-0452-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.
Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.
Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (IHC frozen, PubMed)
Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)
