Contents: Allophycocyanin (APC) anti-human CD43 (Leukosialin, Sialophorin)
Catalog Number: 17-0439
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio84-3C1 (84-3C1)
Isotype: Mouse IgG1
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Staining of normal human peripheral blood cells with FITC anti-human CD3 (UCHT1) (cat. 11-0038) and 0.25 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (left) or APC anti-human CD43 (84-3C1) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood monocytes. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
References
Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
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Cat.
17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart