rat CD25 antibody, Allophycocyanin (APC) conjugate

Contents: Allophycocyanin (APC) anti-rat CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra)
Catalog Number: 17-0390
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OX39
Isotype: Mouse IgG1

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17-0390-82

100 ug

Staining of Lou rat splenocytes with FITC anti-rat CD4 (OX35) (cat. 11-0040) and 0.06 μg of APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (left) or 0.06 μg of APC anti-rat CD25 (OX39) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0390	Affinity Purified anti-rat CD25	N/A	N/A	FC
13-0390	Biotin anti-rat CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra)	N/A	N/A	FC
12-0390	Phycoerythrin (PE) anti-rat CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra)	488	575	FC
17-0390	Allophycocyanin (APC) anti-rat CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra)	633	660	FC IHC(Paraffin)
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OX39 monoclonal antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineages as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OX39 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This OX39 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Paterson DJ, Jefferies WA, Green JR, Brandon MR, Corthesy P, Puklavec M, Williams AF. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987 Dec;24(12):1281-90. (OX39, mAb development, PubMed)

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Cat. 17-0251  APC anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (clone PC61.5)
Cat. 17-0259  Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) (clone BC96)
Cat. 17-4714  Allophycocyanin (APC) Mouse IgG1, K Isotype Control
Cat. 12-5773  PE anti-mouse/rat Foxp3 (clone FJK-16s)