Product Information

Contents: Allophycocyanin (APC) anti-human CD7
Catalog Number: 17-0078
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4H9
Isotype: Mouse IgG2a, k

Staining of normal human peripheral blood cells with PE anti-human CD4 (OKT4) (cat. 12-0048) and 0.125 μg of PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or APC anti-human CD7 (4H9) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0078	Fluorescein isothiocyanate (FITC) anti-human CD7	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.

CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin’s lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin’s lymphoma.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4H9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Seegmiller AC, Karandikar NJ, Kroft SH, McKenna RW, Xu Y. Overexpression of CD7 in classical Hodgkin lymphoma-infiltrating T lymphocytes. Cytometry B Clin Cytom. 2008 Oct 27. Epub ahead of print. (Pubmed)

Weiss LM, Crabtree GS, Rouse RV, Warnke RA. Morphologic and immunologic characterization of 50 peripheral T-cell lymphomas. Am J Pathol. 1985 Feb;118(2): 316-24.

Link M, Warnke R, Finlay J, Amylon M, Miller R, Dilley J, Levy R. A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. Blood. 1983 Oct;62(4): 722-8. (4H9, FC, Pubmed)

Related Products

Cat. 12-0048 Phycoerythrin (PE) anti-human CD4 (clone OKT4 (OKT-4))
Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control