Staining of normal human peripheral blood cells with PE anti-human CD4 (OKT4) (cat. 12-0048) and APC Mouse IgG2a, K Iso Cntrl (cat. 17-4724) (left) or APC anti-human CD7 (4H9) (right). Cells in the lymphocyte gate were used for analysis.
Contents: Allophycocyanin (APC) anti-human CD7 Catalog Number: 17-0078 Concentration: 5 µl (0.25 µg) Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: 4H9 Host/Isotype: Mouse IgG2a, k
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Size
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17-0078-41
25 tests
17-0078-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.
CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin’s lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin’s lymphoma.
Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 4H9 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 μl (0.25 μg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µl. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Seegmiller AC, Karandikar NJ, Kroft SH, McKenna RW, Xu Y. Overexpression of CD7 in classical Hodgkin lymphoma-infiltrating T lymphocytes. Cytometry B Clin Cytom. 2008 Oct 27. (Pubmed)
Weiss LM, Crabtree GS, Rouse RV, Warnke RA. Morphologic and immunologic characterization of 50 peripheral T-cell lymphomas. Am J Pathol. 1985 Feb;118(2): 316-24.
Link M, Warnke R, Finlay J, Amylon M, Miller R, Dilley J, Levy R. A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. Blood. 1983 Oct;62(4): 722-8. (4H9, FC, Pubmed)
