Contents: Allophycocyanin (APC) anti-mouse CD5 (Ly-1)
Catalog Number: 17-0051
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53-7.3
Isotype: Rat IgG2a, κ
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Surface staining of mouse splenocytes with anti-mouse CD5 (53-7.3) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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Description
The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 53-7.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg. 1979. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. 1980. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152(2): 280-95.
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