Product Information

Contents: Allophycocyanin (APC) anti-human CD4 (L3T4)
Catalog Number: 17-0049
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-T4
Isotype: Mouse IgG1, κ
HLDA No.: IV T114

Staining of normal human peripheral blood cells with APC mouse IgG1 isotype control (cat.17-4719) (open histogram) or APC RPA-T4 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0049	Affinity Purified anti-human CD4	N/A	N/A	FA FC IHC
16-0049	Functional Grade* Purified anti-human CD4	N/A	N/A	FA FC
13-0049	Biotin anti-human CD4	N/A	N/A	FC
11-0049	FITC anti-human CD4	488	518	FC
12-0049	PE anti-human CD4	488	575	FC
15-0049	PE-Cy5 anti-human CD4	488	670	FC
17-0049	APC anti-human CD4	633	660	FC
25-0049	PE-Cy7 anti-human CD4	488	760	FC
45-0049	PerCP-Cy5.5 anti-human CD4 (PE-Cy5.5 replacement)	488	690	FC
95-0049	eFluor^TM 650^NC anti-human CD4	350-500	650	FC
47-0049	Allophycocyanin-eFluor^TM 780 anti-human CD4 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0049	eFluor^TM 450 anti-human CD4 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-T4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control