APC anti-human CD4 (L3T4) antibody: allophycocyanin conjugate

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Contents: Allophycocyanin (APC) anti-human CD4
Catalog Number: 17-0048
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT4 (OKT-4)
Isotype: Mouse IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
17-0048-71	25 tests	$120
17-0048-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with APC Mouse IgG2b Iso Cntrl (cat. 17-4732) (open histogram) or APC anti-human CD4 (OKT4) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0048	Fluorescein isothiocyanate (FITC) anti-human CD4	488	518	FC
12-0048	Phycoerythrin (PE) anti-human CD4	488	575	FC
13-0048	Biotin anti-human CD4	N/A	N/A	FC
14-0048	Affinity Purified anti-human CD4	N/A	N/A	FC IP
16-0048	Functional Grade* Purified anti-human CD4	N/A	N/A	FC IP
17-0048	Allophycocyanin (APC) anti-human CD4	633	660	FC
35-0048	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD4	488	690	FC
51-0048	Alexa Fluor® 647 anti-human CD4	633	668	FC
53-0048	Alexa Fluor® 488 anti-human CD4	488	519	FC
56-0048	Alexa Fluor® 700 anti-human CD4 (APC-Cy5.5 Equivalent)	633	723	FC
57-0048	Pacific Blue® anti-human CD4	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)

Related Products

Cat. 11-0049    FITC anti-human CD4 (clone RPA-T4)
Cat. 12-0049    PE anti-human CD4 (clone RPA-T4)
Cat. 13-0049    Biotin anti-human CD4 (clone RPA-T4)
Cat. 14-0049    Affinity Purified anti-human CD4 (clone RPA-T4)
Cat. 15-0049    PE-Cy5 anti-human CD4 (clone RPA-T4)
Cat. 16-0049    Functional Grade Purified anti-human CD4 (clone RPA-T4)
Cat. 17-0049    APC anti-human CD4 (clone RPA-T4)
Cat. 25-0049    PE-Cy7 anti-human CD4 (clone RPA-T4)
Cat. 27-0049    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD4 (APC-Cy7 Equivalent) (clone RPA-T4)
Cat. 17-4732    Allophycocyanin (APC) Mouse IgG2b Isotype Control