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Allophycocyanin (APC) anti-mouse CD4 (L3T4)

 
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Contents: Allophycocyanin (APC) anti-mouse CD4 (L3T4)
Catalog Number: 17-0041
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GK1.5
Isotype: Rat IgG2b, κ

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17-0041-81 50 ug
17-0041-82 100 ug
17-0041-83 200 ug


   
data image 1
Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 μg of APC anti-mouse CD4 (GK1.5) (colored histogram). Total viable cells were used for analysis.
Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-0041 Affinity Purified anti-mouse CD4 (L3T4) N/A N/A FC  IH/F  IP 
16-0041 Functional Grade* Purified anti-mouse CD4 (L3T4) N/A N/A FA  FC 
13-0041 Biotin anti-mouse CD4 (L3T4) N/A N/A FC 
11-0041 FITC anti-mouse CD4 (L3T4) 488 518 FC 
12-0041 PE anti-mouse CD4 (L3T4) 488 575 FC 
15-0041 PE-Cy5 anti-mouse CD4 (L3T4) 488 670 FC 
17-0041 APC anti-mouse CD4 (L3T4) 633 660 FC 
19-0041 Cy5 anti-mouse CD4 (L3T4) 633 670 FC 
25-0041 PE-Cy7 anti-mouse CD4 (L3T4) 488 760 FC 
36-0041 Functional Grade* Biotin anti-mouse CD4 (L3T4) N/A N/A FC  IH/F  IP 
51-0041 Alexa Fluor® 647 anti-mouse CD4 (L3T4) 633 668 FC 
53-0041 Alexa Fluor® 488 anti-mouse CD4 (L3T4) 488 519 FC 
56-0041 Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent) 633 723 FC 
93-0041 eFluorTM 605NC anti-mouse CD4 (L3T4) 350-500 605 FC 
95-0041 eFluorTM 650NC anti-mouse CD4 (L3T4) 350-500 650 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.


Description


The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The GK1.5 antibody has been reported for use in flow cytometric analysis.


Applications Tested


The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References


Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed)

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.


Related Products


Cat. 11-0042  FITC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 12-0042  PE anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 13-0042  Biotin anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 14-0042  Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 15-0042  PE-Cy5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 16-0042  Functional Grade Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-0042  APC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 25-0042  PE-Cy7 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 35-0042  PE-Cy5.5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-4031  Allophycocyanin (APC) Rat IgG2b Isotype Control


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