Product Information

Contents: Allophycocyanin (APC) anti-rat CD4
Catalog Number: 17-0040
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OX35
Isotype: Mouse IgG2a

Staining of rat splenocytes with PE anti-rat CD3 (G4.18) (cat. 12-0030) and 0.125 μg of APC Mouse IgG2a, K Iso Cntrl (cat. 17-4724) (left) or 0.125 μg of APC anti-rat CD4 (OX35) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0040	Affinity Purified anti-rat CD4	N/A	N/A	FC IH/F
11-0040	Fluorescein isothiocyanate (FITC) anti-rat CD4	488	518	FC
17-0040	Allophycocyanin (APC) anti-rat CD4	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OX35 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD. CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170(2):1099-105. (OX35, FC, PubMed)

Related Products

Cat. 17-0041  APC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 17-0042  APC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-0048  Allophycocyanin (APC) anti-human CD4 (clone OKT4 (OKT-4))
Cat. 17-0049  APC anti-human CD4 (clone RPA-T4)
Cat. 17-4724  Allophycocyanin (APC) Mouse IgG2a, K Isotype Control