Product Information

Contents: Allophycocyanin (APC) anti-mouse CD3e
Catalog Number: 17-0031
Sizes: 10 ug, 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 145-2C11
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 splenocytes with PE anti-CD45R/B220 (cat.12-0452) and APC anti-mouse CD3e (145-2C11). Total viable cells were used for analysis. Quadrants were set based on the autofluorescence sample.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0031	FITC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	518	FC
12-0031	PE Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	575	FC
13-0031	Biotin Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FC
14-0031	Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
15-0031	PE-Cy5 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	670	FC
16-0031	Functional Grade* Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC
17-0031	APC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	633	660	FC
25-0031	PE-Cy7 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	760	FC
35-0031	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
36-0031	Functional Grade* Biotin anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
45-0031	PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e)	488	690	FC
53-0031	Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Geng H, Zhang GM, Xiao H, Yuan Y, Li D, Zhang H, Qiu H, He YF, Feng ZH. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer. 2006 Jun 1;118(11):2657-64. (145-2C11, IHC frozen, PubMed)

Krieg C, Han P, Stone R, Goularte OD, Kaye J. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol. 2005 Nov 15;175(10):6420-7. (145-2C11, FA, PubMed)

Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M. CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response. Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3753-60. (145-2C11, IHC frozen, PubMed)

Schuchert MJ, Wright RD, Colson YL. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (145-2C11, WB and IP)

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (145-2C11, IP and WB)

Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14.

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8.

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