Product Information

Contents: Allophycocyanin (APC) anti-human CD2 (LFA-2)
Catalog Number: 17-0029
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-2.10
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Staining of normal human peripheral blood cells with APC Mouse IgG1, K Iso Cntrl (cat. 17-4714) (open histogram) or APC anti-human CD2 (RPA-2.10) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0029	FITC anti-human CD2 (LFA-2)	488	518	FC
12-0029	PE anti-human CD2 (LFA-2)	488	575	FC
13-0029	Biotin anti-human CD2 (LFA-2)	N/A	N/A	FC
14-0029	Affinity Purified anti-human CD2 (LFA-2)	N/A	N/A	FA FC IH/F WB
15-0029	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)	488	670	FC
16-0029	Functional Grade* Purified anti-human CD2 (LFA-2)	N/A	N/A	FC
17-0029	Allophycocyanin (APC) anti-human CD2 (LFA-2)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RPA-2.10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

Related Products

Cat. 17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control