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Anti-Human CD184 (CXCR4) Functional Grade Purified

Also known as: Fusin

RUO: For Research Use Only
Human CXCR4 antibody: anti-Human CXCR4 (Fusin) antibodyStaining of normal human peripheral blood cells with Anti-Human CD184 (CXCR4) PE-Cy5. Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.
Contents: Anti-Human CD184 (CXCR4) Functional Grade Purified
Catalog Number: 16-9999
Concentration: 1 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use contents in sterile envrioment.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 12G5
Host/Isotype: Mouse IgG2a, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
16-9999-81 50 ug
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16-9999-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
16-4724 Mouse IgG2a K Isotype Control Functional Grade Purified
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
12-9999 Anti-Human CD184 (CXCR4) PE 488 575 FC RUO
13-9999 Anti-Human CD184 (CXCR4) Biotin N/A N/A FC RUO
14-9999 Anti-Human CD184 (CXCR4) Purified N/A N/A FA FC RUO
15-9999 Anti-Human CD184 (CXCR4) PE-Cy5 488 670 FC RUO
16-9999 Anti-Human CD184 (CXCR4) Functional Grade* Purified N/A N/A FA FC RUO
17-9999 Anti-Human CD184 (CXCR4) APC 633 660 FC RUO
25-9999 Anti-Human CD184 (CXCR4) PE-Cy7 488 760 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The 12G5 monoclonal antibody reacts with human CXCR4 (CD184), also termed Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. 12G5 shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CXCR4), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies.
Applications Tested
The 12G5 antibody has been tested by flow cytometric analysis of human peripheral leukocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References

McKnight, A. et al. (1997). "Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent." J Virol 71(2): 1692-6.
Bleul, C. et al. (1997). "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." Proc Natl Acad Sci U S A 94(5): 1925-30.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.