Surface staining of normal human peripheral blood cells with anti-human CXCR4 (12G5) PE-Cy5. Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.
Contents: Functional Grade Purified anti-human CXCR4 (Fusin, CD184) Catalog Number: 16-9999 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Clone: 12G5 Host/Isotype: Mouse IgG2a, κ Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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16-9999-81
50 ug
16-9999-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The 12G5 monoclonal antibody reacts with human CXCR4 (CD184), also termed Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. 12G5 shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CXCR4), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies.
Applications Tested
The 12G5 antibody has been tested by flow cytometric analysis of human peripheral leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
McKnight, A. et al. (1997). "Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent." J Virol 71(2): 1692-6. Bleul, C. et al. (1997). "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." Proc Natl Acad Sci U S A 94(5): 1925-30.
